Anti-NQO1 Antibody Picoband®

About this product

Boster Bio Anti-NQO1 Antibody Picoband® catalog # PB9497. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9497

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. And this FAD-binding protein forms homodimers and reduces quinones to hydroquinones. In addition, this protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD) . Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Synonyms

NAD (P) H dehydrogenase [quinone] 1;1.6.5.2; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD (P) H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1; NQO1; DIA4, NMOR1

Gene Name

NQO1

Gene ID

1728

UniProt

P15559

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NQO1, different from the related mouse and rat sequences by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

WB

Field of Research

Cancer, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

References & Citations

1. Entrez Gene: NQO1 NAD (P) H dehydrogenase, quinone 1. 2. Jaiswal AK (Nov 1991) . Human NAD (P) H:quinone oxidoreductase (NQO1) gene structure and induction by dioxin.Biochemistry x30 (44) : 10647–53. 3. Ross D, Siegel D (2004) . NAD (P) H:quinone oxidoreductase 1 (NQO1, DT-diaphorase), functions and pharmacogenetics. Methods in Enzymology382: 115–44.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30868 MW

Observed Molecular Weight

31 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NAD (P) H dehydrogenase [quinone] 1

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the NAD (P) H dehydrogenase (quinone) family.

Protein Name

NAD (P) H dehydrogenase [quinone] 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.