Anti-PINX1 Antibody Picoband®

About this product

Boster Bio Anti-PINX1 Antibody Picoband® catalog # PB9498. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9498

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PINX1, also known as PIN2 interacting protein 1, is a telomerase inhibitor and a possible tumor suppressor. It is mapped to 8p23. Over-expression of PINX1 results in decreased telomerase activity, telomere shortening, and induction of crisis. Reduction of PINX1 leads to an increase in telomerase activity and elongation of telomeres. PINX1 differs from other proteins that regulate telomere length in that it acts on telomerase while other proteins adjust telomere length without affecting telomerase activity. The PINX1 budding yeast orthologue Gnop1 inhibits telomerase by isolating the uncomplexed TERT protein so that it cannot associate with the telomerase template RNA, which prevents telomerase from being assembled. However, in humans, PINX1 impedes already assembled telomerase.

Synonyms

PIN2/TERF1-interacting telomerase inhibitor 1; Liver-related putative tumor suppressor; Pin2-interacting protein X1; Protein 67-11-3; TRF1-interacting protein 1; PINX1; LPTL, LPTS

Gene Name

PINX1

Gene ID

54984

UniProt

Q96BK5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PINX1, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.

Applications

WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. .

References & Citations

1. Banik, S.S.R.; Counter, C.M. (September 2004) . Characterization of Interactions between PinX1 and Human Telomerase Subunits hTERT and hTR. Journal of Biological Chemistry. 2. Zhou, X Z; Lu K P (Nov 2001) . The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor. Cell (United States) 107 (3) : 347–59.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

37035 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

PIN2/TERF1-interacting telomerase inhibitor 1

Subcellular Location

Nucleus. Nucleus, nucleolus. Chromosome, telomere. Chromosome, centromere, kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.

Sequence Similarities

Belongs to the PINX1 family.

Protein Name

PIN2/TERF1-interacting telomerase inhibitor 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.