Anti-alpha Sarcoglycan/SGCA Antibody Picoband®

About this product

Boster Bio Anti-alpha Sarcoglycan/SGCA Antibody Picoband® catalog # PB9503. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9503

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-sarcoglycan is a protein that in humans is encoded by the SGCA gene. This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Alpha-sarcoglycan; Alpha-SG;50 kDa dystrophin-associated glycoprotein;50DAG; Adhalin; Dystroglycan-2; SGCA; ADL, DAG2

Gene Name

SGCA

Gene ID

6442

UniProt

Q16586

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SGCA recombinant protein (Position: Q24-H387) . Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA.

Clonality

Polyclonal

Tissue Specificity

Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.

Applications

WB

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

References & Citations

1. Entrez Gene: SGCA sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) . 2. McNally EM, Yoshida M, Mizuno Y, Ozawa E, Kunkel LM (Nov 1994) . Human adhalin is alternatively spliced and the gene is located on chromosome 17q21. Proc Natl Acad Sci U S A 91 (21) : 9690–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42875 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Alpha-sarcoglycan

Subcellular Location

Cell membrane, sarcolemma ; Single-pass type I membrane protein . Cytoplasm, cytoskeleton .

Sequence Similarities

Belongs to the sarcoglycan alpha/epsilon family.

Protein Name

Alpha-sarcoglycan

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.