Anti-SP3 Antibody Picoband®

About this product

Boster Bio Anti-SP3 Antibody Picoband® catalog # PB9508. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9508

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sp3 transcription factor, also known as SP3, refers to both a protein and the gene it is encoded by. This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. And this protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.

Synonyms

Transcription factor Sp3; SPR-2; SP3

Gene Name

SP3

Gene ID

6670

UniProt

Q02447

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SP3, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping. .

References & Citations

1. Entrez Gene: SP3 Sp3 transcription factor. 2. Sun, Jian-Min; Chen Hou Yu; Moniwa Mariko; Litchfield David W; Seto Edward; Davie James R (September 2002) . The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2. J. Biol. Chem. (United States) 277 (39) : 35783–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81925 MW

Observed Molecular Weight

82 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Transcription factor Sp3

Subcellular Location

Nucleus. Nucleus, PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.

Sequence Similarities

Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein Name

Transcription factor Sp3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.