Anti-TNF Receptor II/TNFRSF1B Antibody Picoband®

About this product

Boster Bio Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® catalog # PB9514. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9514

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor receptor 2 (TNFR2) is one of receptors of TNF. TNF has proinflammatory and immunosuppressive properties that may segregate at the level of the 2 TNF receptors (TNFRs), TNFR1 and TNFR2. The genes for TNFR1, a 55-kDa protein, and TNFR2, a 70-kDa protein, have been mapped to human chromosomes 12 (12pter-cen) and 1 (1pter-p32), respectively. TNFR2 was induced on glomerular endothelial cells of nephritic kidneys, and TNFR2 expression on intrinsic cells, but not leukocytes, was essential for glomerulonephritis and glomerular complement deposition. TNFR1 promotes systemic immune responses and renal T cell death, while intrinsic cell TNFR2 plays a critical role in complement-dependent tissue injury. Therefore, therapeutic blockade specifically of TNFR2 may be a promising strategy in the treatment of immune-mediated glomerulonephritis.

Synonyms

Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD120b; Etanercept; Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding protein 2; TBP-2; TBPII; TNFRSF1B; TNFBR, TNFR2

Gene Name

TNFRSF1B

Gene ID

7133

UniProt

P20333

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TNF Receptor II, different from the related mouse sequence by eleven amino acids, and from the related rat sequence by ten amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages. .

Applications

WB

Field of Research

Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Growth Factors/Hormones, Receptors, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. .

References & Citations

1. Milatovich, A.; Song, K.; Heller, R. A.; Francke, U. : Tumor necrosis factor receptor genes, TNFR1 and TNFR2, on human chromosomes 12 and 1. Somat. Cell Molec. Genet. 17: 519-523, 1991. 2. Vielhauer, V.; Stavrakis, G.; Mayadas, T. N. : Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis. J. Clin. Invest. 115: 1199-1209, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48291 MW

Observed Molecular Weight

60-65 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 1B

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 4 TNFR-Cys repeats.

Protein Name

Tumor necrosis factor receptor superfamily member 1B

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.