Anti-CD59 glycoprotein CD59 Antibody Picoband®

About this product

Boster Bio Anti-CD59 glycoprotein CD59 Antibody catalog # RP1076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1076

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. And this protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Synonyms

CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MEM43 antigen; Membrane attack complex inhibition factor; MACIF; Membrane inhibitor of reactive lysis; MIRL; Protectin; CD59; CD59; MIC11, MIN1, MIN2, MIN3, MSK21

Gene Name

CD59

Gene ID

966

UniProt

P13987

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CD59 recombinant protein (Position: L26-N102) . Human CD59 shares 47.1% amino acid (aa) sequence identity with rat CD59.

Clonality

Polyclonal

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Atherosclerosis, Cardiovascular, Cell Type Markers, Complement, Hematopoietic Progenitors, Immunology, Innate Immunity, Protein Phosphorylation, Signal Transduction, Stem Cells, Surface Molecules, Tyrosine Kinases, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

References & Citations

1. Entrez Gene: CD59 molecule, complement regulatory protein. 2. Ninomiya H, Sims PJ (1992) . The human complement regulatory protein CD59 binds to the alpha-chain of C8 and to the bdomain of C9. J. Biol. Chem. 267 (19) : 13675–80.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

14177 MW

Observed Molecular Weight

19 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CD59 glycoprotein

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Soluble form found in a number of tissues.

Sequence Similarities

Contains 1 UPAR/Ly6 domain.

Protein Name

CD59 glycoprotein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.