Anti-BAG2 Antibody Picoband®

About this product

Boster Bio Anti-BAG2 Antibody Picoband® catalog # PB9524. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9524

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.

Synonyms

BAG family molecular chaperone regulator 2; BAG-2; Bcl-2-associated athanogene 2; BAG2

Gene Name

BAG2

Gene ID

9532

UniProt

O95816

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human BAG2 recombinant protein (Position: M1-N211) . Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.

Clonality

Polyclonal

Tissue Specificity

Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level) . Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level) . Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level) . Detected in urine (at protein level) . Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level) . .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.

References & Citations

1. Takayama S, Xie Z, Reed JC (Feb 1999) . An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J Biol Chem 274 (2) : 781–6. 2. Entrez Gene: BAG2 BCL2-associated athanogene 2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23772 MW

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

BAG family molecular chaperone regulator 2

Subcellular Location

Secreted .

Sequence Similarities

Contains 1 P-type (trefoil) domain.

Protein Name

BAG family molecular chaperone regulator 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.