Anti-Bcl10 Antibody Picoband®

About this product

Boster Bio Anti-Bcl10 Antibody Picoband® catalog # PB9525. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9525

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BCL10, B-cell CLL/lymphoma 10, gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The BCL10 gene is mapped to chromosome 1p22. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. And this protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.

Synonyms

B-cell lymphoma/leukemia 10; B-cell CLL/lymphoma 10; Bcl-10; CARD-containing molecule enhancing NF-kappa-B; CARD-like apoptotic protein; hCLAP; CED-3/ICH-1 prodomain homologous E10-like regulator; CIPER; Cellular homolog of vCARMEN; cCARMEN; Cellular-E10; c-E10; Mammalian CARD-containing adapter molecule E10; mE10; BCL10; CIPER, CLAP

Gene Name

BCL10

Gene ID

8915

UniProt

O95999

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Bcl10 recombinant protein (Position: M1-Q233) . Human Bcl10 shares 91% and 91.4% amino acid (aa) sequence identity with mouse and rat Bcl10, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IHC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, NFKB Pathway, Nuclear Signaling, Pathways and Processes, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in adaptive immune response (PubMed:25365219) . Promotes apoptosis, pro-caspase-9 maturation and activation of NF- kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1 (PubMed:18264101) . .

References & Citations

1. Ruland, J., Duncan, G. S., Elia, A., del Barco Barrantes, I., Nguyen, L., Plyte, S., Millar, D. G., Bouchard, D., Wakeham, A., Ohashi, P. S., Mak, T. W.Bcl10 is a positive regulator of antigen receptor-induced activation of NF-kappa-B and neural tube closure.Cell 104: 33-42, 2001. 2. Willis, T. G., Jadayel, D. M., Du, M.-Q., Peng, H., Perry, A. R., Abdul-Rauf, M., Price, H., Karran, L., Majekodunmi, O., Wlodarska, I., Pan, L., Crook, T., Hamoudi, R., Isaacson, P. G., Dyer, M. J. S.Bcl10 is involved in t (1;14) (p22; q32) of MALT B cell lymphoma and mutated in multiple tumor typesCell 96: 35-45, 1999. 3. Xue, L., Morris, S. W., Orihuela, C., Tuomanen, E., Cui, X., Wen, R., Wang, D.Defective development and function of Bcl10-deficient follicular, marginal zone and B1 B cells.Nature Immun. 4: 857-865, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26252 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

B-cell lymphoma/leukemia 10

Subcellular Location

Cytoplasm, perinuclear region . Membrane raft . Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.

Sequence Similarities

Contains 1 CARD domain.

Protein Name

B-cell lymphoma/leukemia 10

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.