- Catalog number
- PB9527
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Baculoviral IAP repeat-containing protein 3 (also known as cIAP2) is a protein that in humans is encoded by the BIRC3 gene. cIAP2 is a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified.
- Synonyms
- Baculoviral IAP repeat-containing protein 3;6.3.2.-; Apoptosis inhibitor 2; API2; C-IAP2; IAP homolog C; Inhibitor of apoptosis protein 1; IAP-1; hIAP-1; hIAP1; RING finger protein 49; TNFR2-TRAF-signaling complex protein 1; BIRC3; API2, IAP1, MIHC, RNF49
- Gene Name
- BIRC3
- Gene ID
- 330
- UniProt
- Q13489
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human cIAP2 recombinant protein (Position: M1-K191) . Human cIAP2 shares 69.8% amino acid (aa) sequence identity with mouse cIAP2.
- Clonality
- Polyclonal
- Tissue Specificity
- Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
- Applications
- WB
- Field of Research
- Alzheimer's Disease, Drug Metabolism, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Neurotransmitter, Pathways and Processes, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite ions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs) . Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase- independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. .
- References & Citations
- 1. Entrez Gene: BIRC3 baculoviral IAP repeat-containing 3. 2. Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A, Korneluk RG (February 1996) . Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes. Nature 379 (6563) : 349–53. 3. Rothe M, Pan MG, Henzel WJ, Ayres TM, Goeddel DV (February 1996) . The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 83 (7) : 1243–52.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 68372 MW
- Observed Molecular Weight
- 68 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Baculoviral IAP repeat-containing protein 3
- Subcellular Location
- Cytoplasm . Nucleus .
- Sequence Similarities
- Belongs to the IAP family.
- Protein Name
- Baculoviral IAP repeat-containing protein 3
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
