Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband®

About this product

Boster Bio Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband® catalog # PB9544. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9544

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline) . It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase) . It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo (a) pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo (a) pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo (a) pyrene carcinogen into the systemic circulation.

Synonyms

Cytochrome P450 1A1;1.14.14.1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A1

Gene Name

CYP1A1

Gene ID

1543

UniProt

P04798

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320) . Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.

Clonality

Polyclonal

Tissue Specificity

Lung, lymphocytes and placenta.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial Metabolism, Pathways and Processes, Response To Hypoxia, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

References & Citations

1. Beresford AP (1993) . CYP1A1: friend or foe?. Drug Metabolism Reviews 25 (4) : 503–17. 2. Uno, S (2004) . Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible cytochrome P450 is more important than metabolic activation.. Mol Pharmacol 65 (5) : 1225–37.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

58 kDa

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cytochrome P450 1A1

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Sequence Similarities

Belongs to the cytochrome P450 family.

Protein Name

Cytochrome P450 1A1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.