Anti-CYP1B1 Antibody Picoband®

About this product

Boster Bio Anti-CYP1B1 Antibody Picoband® catalog # PB9546. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9546

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytochrome P450 1B1 is an enzyme that in humans is encoded by the CYP1B1 gene. CYP1B1 belongs to the the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.

Synonyms

Cytochrome P450 1B1;1.14.14.1; CYPIB1; CYP1B1

Gene Name

CYP1B1

Gene ID

1545

UniProt

Q16678

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CYP1B1 recombinant protein (Position: R255-L480) . Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in many tissues. .

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cardiovascular, Cholesterol Metabolism, Drug Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neuroscience, Pathways and Processes, Sensory System, Signal Transduction, Visual System

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta- estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression. .

References & Citations

1. Entrez Gene: cytochrome P450. 2. Bailey LR, Roodi N, Dupont WD, Parl FF (1998) . Association of cytochrome P450 1B1 (CYP1B1) polymorphism with steroid receptor status in breast cancer.. Cancer Res. 58 (22) : 5038–41. 3. Michels-Rautenstrauss KG, Mardin CY, Zenker M et al. (2002) . Primary congenital glaucoma: three case reports on novel mutations and combinations of mutations in the GLC3A (CYP1B1) gene.. J. Glaucoma 10 (4) : 354–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60846 MW

Observed Molecular Weight

61 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cytochrome P450 1B1

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion .

Sequence Similarities

Belongs to the cytochrome P450 family.

Protein Name

Cytochrome P450 1B1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.