Anti-CYP24A1 Antibody Picoband®

About this product

Boster Bio Anti-CYP24A1 Antibody Picoband® catalog # PB9547. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9547

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. And this mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial;24-OHase; Vitamin D (3) 24-hydroxylase;1.14.13.126; Cytochrome P450 24A1; Cytochrome P450-CC24; CYP24A1; CYP24

Gene Name

CYP24A1

Gene ID

1591

UniProt

Q07973

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CYP24A1 recombinant protein (Position: E153-R514) . Human CYP24A1 shares 86.2% amino acid (aa) sequence identity with both mouse and rat CYP24A1.

Clonality

Polyclonal

Tissue Specificity

Expressed in many tissues. .

Applications

IHC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Cofactors, Vitamins/Minerals, Cytochromes, Endocrine Metabolism, Hormone Biosynthesis, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D (3) ) and calcitriol (1-alpha,25-dihydroxyvitamin D (3) ) . The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D (3) -26,23-lactone as end product.

References & Citations

1. Entrez Gene: CYP24A1 cytochrome P450, family 24, subfamily A, polypeptide 1. 2. Dauber A, Nguyen TT, Sochett E, Cole DE, Horst R, Abrams SA, Carpenter TO, Hirschhorn JN (February 2012) . Genetic defect in CYP24A1, the vitamin D 24-hydroxylase gene, in a patient with severe infantile hypercalcemia. J. Clin. Endocrinol. Metab. 97 (2) : E268–74. 3. Sakaki T, Kagawa N, Yamamoto K, Inouye K (January 2005) . Metabolism of vitamin D3 by cytochromes P450. Front. Biosci. 10: 119–34.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59 kDa

Observed Molecular Weight

59 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial

Subcellular Location

Mitochondrion.

Sequence Similarities

Belongs to the cytochrome P450 family.

Protein Name

1,25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.