Anti-CYP27B1 Antibody Picoband®

About this product

Boster Bio Anti-CYP27B1 Antibody Picoband® catalog # PB9548. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9548

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Synonyms

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;1.14.13.13;25-OHD-1 alpha-hydroxylase;25-hydroxyvitamin D (3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1; CYP27B1; CYP1ALPHA, CYP27B

Gene Name

CYP27B1

Gene ID

1594

UniProt

O15528

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids.

Clonality

Polyclonal

Tissue Specificity

Kidney.

Applications

IHC, WB

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Cofactors, Vitamins/Minerals, Cytochromes, Endocrine Metabolism, Hormone Biosynthesis, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the conversion of 25-hydroxyvitamin D3 (25 (OH) D) to 1-alpha,25-dihydroxyvitamin D3 (1,25 (OH) 2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

References & Citations

1. Entrez Gene: cytochrome P450. 2. Monkawa T, Yoshida T, Wakino S, Shinki T, Anazawa H, Deluca HF et al. (Oct 1997) . Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1 alpha-hydroxylase. Biochemical and Biophysical Research Communications 239 (2) : 527–33. 3. Takeyama K, Kitanaka S, Sato T, Kobori M, Yanagisawa J, Kato S (Sep 1997) . 25-Hydroxyvitamin D3 1alpha-hydroxylase and vitamin D synthesis. Science (New York, N.Y.) 277 (5333) : 1827–30.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56504 MW

Observed Molecular Weight

57 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Subcellular Location

Mitochondrion membrane.

Sequence Similarities

Belongs to the cytochrome P450 family.

Protein Name

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.