Anti-DKK2 Antibody Picoband®

About this product

Boster Bio Anti-DKK2 Antibody Picoband® catalog # PB9551. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9551

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dickkopf-related protein 2 is a protein that in humans is encoded by the DKK2 gene. This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6) .

Synonyms

Dickkopf-related protein 2; Dickkopf-2; Dkk-2; hDkk-2; DKK2; UNQ682/PRO1316

Gene Name

DKK2

Gene ID

27123

UniProt

Q9UBU2

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human DKK2 recombinant protein (Position: K34-I259) . Human DKK2 shares 96% amino acid (aa) sequence identity with mouse DKK2.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, skeletal muscle and lung.

Applications

WB

Field of Research

Secreted, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity) . .

References & Citations

1. Entrez Gene: DKK2 dickkopf homolog 2 (Xenopus laevis) . 2. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA (Dec 1999) . Functional and structural diversity of the human Dickkopf gene family. Gene 238 (2) : 301–13. 3. Wu W, Glinka A, Delius H, Niehrs C (2001) . Mutual antagonism between dickkopf1 and dickkopf2 regulates Wnt/beta-catenin signalling.. Curr. Biol. 10 (24) : 1611–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28447 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Dickkopf-related protein 2

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the DAXX family.

Protein Name

Dickkopf-related protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.