Anti-E Cadherin 1/CDH1 Antibody Picoband®

About this product

Boster Bio Anti-E Cadherin 1/CDH1 Antibody Picoband® catalog # PB9561. Tested in ELISA, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9561

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter) . Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Synonyms

Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; CDH1; CDHE, UVO

Gene Name

CDH1

Gene ID

999

UniProt

P12830

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703) . Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

Clonality

Polyclonal

Tissue Specificity

Non-neural epithelial tissues.

Applications

ELISA, ICC/IF, IHC, WB

Field of Research

Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. .

References & Citations

1. Agiostratidou G., Muros R.M., Shioi J., Marambaud P., Robakis N.K. The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors. J. Neurochem. 96:1182-1188 (2006) . 2. Frebourg, T., Oliveira, C., Hochain, P., Karam, R., Manouvrier, S., Graziadio, C., Vekemans, M., Hartmann, A., Baert-Desurmont, S., Alexandre, C., Lejeune Dumoulin, S., Marroni, C., and 16 others. Cleft lip/palate and CDH1/E-cadherin mutation in families with hereditary diffuse gastric cancer. J. Med. Genet. 43: 138-142, 2006. 3. Mansouri, A., Goodfellow, P. N., Kemler, R. Molecular cloning and chromosomal localization of the human cell adhesion molecule uvomorulin (UVO) . (Abstract) Cytogenet. Cell Genet. 46: 655, 1987. 4. Mansouri, A., Spurr, N., Goodfellow, P. N., Kemler, R. Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin. Differentiation 38: 67-71, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

98 kDa

Observed Molecular Weight

130 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cadherin-1

Subcellular Location

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Sequence Similarities

Contains 5 cadherin domains.

Protein Name

Cadherin-1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.