- Catalog number
- PB9562
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
- Synonyms
- Macrophage colony-stimulating factor 1; CSF-1; MCSF; Processed macrophage colony-stimulating factor 1; Csf1; Csfm
- Gene Name
- Csf1
- Gene ID
- 12977
- UniProt
- P07141
- Host
- Rabbit
- Reactivity
- Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E. coli-derived mouse MCSF recombinant protein (Position: K33-E262) . Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Non-neural epithelial tissues.
- Applications
- ELISA, IHC
- Field of Research
- Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
- References & Citations
- 1. Fixe P, Praloran V (June 1997) .Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. Eur. Cytokine Netw. 8 (2) : 125–36. 2. Nemunaitis J (April 1993) . Macrophage function activating cytokines: potential clinical application. Crit. Rev. Oncol. Hematol. 14 (2) : 153–71. 3. Stanley ER, Berg KL, Einstein DB, Lee PS, Pixley FJ, Wang Y, Yeung YG (January 1997) . Biology and action of colony--stimulating factor-1. Mol. Reprod. Dev. 46 (1) : 4–10.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 60649 MW
- Observed Molecular Weight
- 130 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Macrophage colony-stimulating factor 1
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Sequence Similarities
- Contains 5 cadherin domains.
- Protein Name
- Macrophage colony-stimulating factor 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
