Anti-CYP7A1 Antibody Picoband®

About this product

Boster Bio Anti-CYP7A1 Antibody catalog # RP1079. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1079

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA -HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.

Synonyms

Cholesterol 7-alpha-monooxygenase;1.14.13.17; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1; CYP7A1; CYP7

Gene Name

CYP7A1

Gene ID

1581

UniProt

P22680

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504) . Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in liver. .

Applications

Flow Cytometry, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cell Differentiation, Epigenetics and Nuclear Signaling, Host-Virus Interaction, Interspecies Interaction, Microbiology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. .

References & Citations

1. Agellon, L. B., Drover, V. A. B., Cheema, S. K., Gbaguidi, G. F., Walsh, A. Dietary cholesterol fails to stimulate the human cholesterol 7-alpha-hydroxylase gene (CYP7A1) in transgenic mice. J. Biol. Chem. 277: 20131-20134, 2002. 2. Cohen, J. C., Cali, J. J., Jelinek, D. F., Mehrabian, M., Sparkes, R. S., Lusis, A. J., Russell, D. W., Hobbs, H. H. Cloning of the human cholesterol 7-alpha-hydroxylase gene (CYP7) and localization to chromosome 8q11-q12. Genomics 14: 153-161, 1992. 3. Molowa, D. T., Chen, W. S., Cimis, G. M., Tan, C. P. Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene. Biochemistry 31: 2539-2544, 1992.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57661 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cholesterol 7-alpha-monooxygenase

Subcellular Location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Sequence Similarities

Belongs to the cytochrome P450 family.

Protein Name

Cholesterol 7-alpha-monooxygenase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.