Anti-Eotaxin/CCL11 Antibody Picoband®

About this product

Boster Bio Anti-Eotaxin/CCL11 Antibody Picoband® catalog # PB9569. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9569

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCL11 (Chemokine (C-C motif) ligand 11), also known as Eotaxin, is a small cytokine belonging to the CC chemokine family. Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which Eotaxin is a ligand include CCR2, CCR3 and CCR5. However, it has been found that eotaxin-1 (CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3. The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12.

Synonyms

Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11; CCL11; SCYA11

Gene Name

CCL11

Gene ID

6356

UniProt

P51671

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived human Eotaxin recombinant protein (Position: G24-P97) . Human Eotaxin shares 61.6% and 63% amino acid (aa) sequence identity with mouse and rat Eotaxin, respectively.

Clonality

Polyclonal

Tissue Specificity

Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.

Applications

ELISA, IHC, WB

Field of Research

Chemokines, Immunology, Innate Immunity

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.

References & Citations

1. Menzies-Gow, A., Ying, S., Sabroe, I., Stubbs, V. L., Soler, D., Williams, T. J., Kay, A. B.Eotaxin (CCL11) and eotaxin-2 (CCL24) induce recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteers.J. Immun. 169: 2712-2718, 2002. 2. Shin, H. D., Kim, L. H., Park, B. L., Jung, J. H., Kim, J. Y., Chung, I.-Y., Kim, J. S., Lee, J. H., Chung, S. H., Kim, Y. H., Park, H.-S., Choi, J. H., Lee, Y. M., Park, S. W., Choi, B. W., Hong, S.-J., Park, C.-S.Association of eotaxin gene family with asthma and serum total IgE.Hum. Molec. Genet. 12: 1279-1285, 2003. Note: Erratum: Hum. Molec. Genet. 12: 2083 only, 2003. 3. Villeda, S. A., Luo, J., Mosher, K. I., Zou, B., Britschgi, M., Bieri, G., Stan, T. M., Fainberg, N., Ding, Z., Eggel, A., Lucin, K. M., Czirr, E., and 11 others.The ageing systemic milieu negatively regulates neurogenesis and cognitive function.Nature 477: 90-94, 2011.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10732 MW

Observed Molecular Weight

11, 15 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Eotaxin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

Eotaxin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.