Anti-Isocitrate dehydrogenase/IDH1 Antibody Picoband®

About this product

Boster Bio Anti-Isocitrate dehydrogenase/IDH1 Antibody Picoband® catalog # PB9601. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9601

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Isocitrate dehydrogenase 1 (NADP+), soluble is an enzyme that in humans is encoded by the IDH1 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Synonyms

Isocitrate dehydrogenase [NADP] cytoplasmic; IDH;1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP (+) -specific ICDH; Oxalosuccinate decarboxylase; IDH1; PICD

Gene Name

IDH1

Gene ID

3417

UniProt

O75874

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IDH1, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References & Citations

1. Entrez Gene: Isocitrate dehydrogenase 1 (NADP+), soluble. 2. Watanabe T, Nobusawa S, Kleihues P, Ohgaki H (April 2009) . IDH1 mutations are early events in the development of astrocytomas and oligodendrogliomas.. Am J Pathol. 174 issue = 4 (4) : 1149–53.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46659 MW

Observed Molecular Weight

47 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Isocitrate dehydrogenase [NADP] cytoplasmic

Subcellular Location

Cytoplasm . Peroxisome .

Sequence Similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Protein Name

Isocitrate dehydrogenase [NADP] cytoplasmic

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.