Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband®

About this product

Boster Bio Anti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband® catalog # PB9603. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9603

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

IDO1 (INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.

Synonyms

Indoleamine 2,3-dioxygenase 1; IDO-1;1.13.11.52; Indoleamine-pyrrole 2,3-dioxygenase; IDO1; IDO, INDO

Gene Name

IDO1

Gene ID

3620

UniProt

P14902

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human IDO1, different from the related mouse sequence by fourteen amino acids, and from the related rat sequence by seventeen amino acids.

Clonality

Polyclonal

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Atherosclerosis, Cancer, Cardiovascular, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. .

References & Citations

1. Burkin, D. J., Kimbro, K. S., Barr, B. L., Jones, C., Taylor, M. W., Gupta, S. L. Localization of the human indoleamine 2,3-dioxygenase (IDO) gene to the pericentromeric region of human chromosome 8. Genomics 17: 262-263, 1993. 2. Dai, W., Gupta, S. L. Molecular cloning, sequencing and expression of human interferon-gamma-inducible indoleamine 2,3-dioxygenase cDNA. Biochem. Biophys. Res. Commun. 168: 1-8, 1990. 3. Fallarino, F., Grohmann, U., Hwang, K. W., Orabona, C., Vacca, C., Bianchi, R., Belladonna, M. L., Fioretti, M. C., Alegre, M.-L., Puccetti, P. Modulation of tryptophan catabolism by regulatory T cells. Nature Immun. 4: 1206-1212, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

45326 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Indoleamine 2,3-dioxygenase 1

Subcellular Location

Mitochondrion.

Sequence Similarities

Belongs to the indoleamine 2,3-dioxygenase family.

Protein Name

Indoleamine 2,3-dioxygenase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.