Anti-MICB Antibody Picoband®

About this product

Boster Bio Anti-MICB Antibody Picoband® catalog # PB9613. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9613

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MHC class I polypeptide-related sequence B is a protein that in humans is encoded by the MICB gene. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants.

Synonyms

MHC class I polypeptide-related sequence B; MIC-B; MICB ; PERB11.2

Gene Name

MICB

Gene ID

4277

UniProt

Q29980

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MICB.

Clonality

Polyclonal

Tissue Specificity

Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. .

Applications

Flow Cytometry, WB

Field of Research

Adaptive Immunity, Class I, Immunology

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. .

References & Citations

1. Bahram S, Bresnahan M, Geraghty DE, Spies T (Aug 1994) . A second lineage of mammalian major histocompatibility complex class I genes. Proc Natl Acad Sci U S A 91 (14) : 6259–63. 2. Entrez Gene: MICB MHC class I polypeptide-related sequence B. 3. Leelayuwat C, Townend DC, Degli-Esposti MA et al. (1994) . A new polymorphic and multicopy MHC gene family related to nonmammalian class I..Immunogenetics 40 (5) : 339–51.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42646 MW

Observed Molecular Weight

60-65 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

MHC class I polypeptide-related sequence B

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. .

Sequence Similarities

Belongs to the MHC class I family. MIC subfamily.

Protein Name

MHC class I polypeptide-related sequence B

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.