Anti-Wnt3 Antibody Picoband®

About this product

Boster Bio Anti-Wnt3 Antibody Picoband® catalog # PB9619. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9619

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Synonyms

Proto-oncogene Wnt-3; Proto-oncogene Int-4 homolog; WNT3; INT4

Gene Name

WNT3

Gene ID

7473

UniProt

P56703

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Wnt3, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Testis, brain, and heart.

Applications

WB

Field of Research

Neurogenesis, Neurology Process, Neuroscience, Signaling Pathways, Stem Cells, Surface Molecules

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube (By similarity) . .

References & Citations

1. Entrez Gene: WNT3 wingless-type MMTV integration site family, member 3. 2. Liu P, Wakamiya M, Shea MJ et al. (1999) . Requirement for Wnt3 in vertebrate axis formation.Nat. Genet. 22 (4) : 361–5. 3. Roelink H, Wang J, Black DM, Solomon E, Nusse R (Dec 1993) . Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17 (3) : 790–2.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39645 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Proto-oncogene Wnt-3

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Proto-oncogene Wnt-3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.