Anti-DMRT1 Antibody Picoband®

About this product

Boster Bio Anti-DMRT1 Antibody catalog # RP1083. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1083

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain) . The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Synonyms

Doublesex- and mab-3-related transcription factor 1; DM domain expressed in testis protein 1; DMRT1; DMT1

Gene Name

DMRT1

Gene ID

1761

UniProt

Q9Y5R6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Testis-specific. Expressed in prostate cancer (at protein level) . .

Applications

IHC, WB

Field of Research

Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA) -dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis (By similarity) . .

References & Citations

1. Entrez Gene: DMRT1 doublesex and mab-3 related transcription factor 1. 2. Raymond CS, Shamu CE, Shen MM, Seifert KJ, Hirsch B, Hodgkin J, Zarkower D (February 1998) . Evidence for evolutionary conservation of sex-determining genes.Nature 391 (6668) : 691–5. 3. Smith CA, Roeszler KN, Ohnesorg T, Cummins DM, Farlie PG, Doran TJ, Sinclair AH (August 2009) . The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature 461 (7261) : 267–71.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39473 MW

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Doublesex- and mab-3-related transcription factor 1

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the DMRT family.

Protein Name

Doublesex- and mab-3-related transcription factor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.