Anti-ERVW-1 Antibody Picoband®

About this product

Boster Bio Anti-ERVW-1 Antibody Picoband® catalog # PB9622. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9622

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ERVW-1 is also known as ERVWE1. The human ERVWE1 locus is derived from a human endogenous retrovirus-W (HERV-W) provirus located on chromosome 7. This provirus has inactivating mutations in the gag and pol genes, but the envelope glycoprotein gene has been selectively preserved. The product of this gene, syncytin, is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer.

Synonyms

Syncytin-1; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin; Surface protein; SU; gp50; Transmembrane protein; TM; gp24; ERVW-1; ERVWE1

Gene Name

ERVW-1

Gene ID

30816

UniProt

Q9UQF0

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ERVW-1.

Clonality

Polyclonal

Tissue Specificity

Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis. Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and trachea. Both mRNA and protein levels are significantly increased in the brain of individuals with multiple sclerosis, particularly in astrocytes and microglia. .

Applications

WB

Field of Research

Developmental Biology, Reproduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5 (PubMed:10708449, PubMed:12050356, PubMed:23492904) . .

References & Citations

1. Entrez Gene: ERVWE1 endogenous retroviral family W, env (C7), member 1 (syncytin) . 2. Li F, Nellåker C, Sabunciyan S, Yolken RH, Jones-Brando L, Johansson AS, Owe-Larsson B, Karlsson H. “Transcriptional derepression of the ERVWE1 locus following influenza A virus infection.” J Virol, 2014 Apr.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

59866 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Syncytin-1

Subcellular Location

Transmembrane protein: Cell membrane ; Single-pass type I membrane protein .

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Syncytin-1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.