Anti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband®

About this product

Boster Bio Anti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband® catalog # PB9627. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9627

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver (hepatocytes) and kidney (proximal tubules) . In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity, thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Synonyms

Glutathione S-transferase A1; 2.5.1.18; GST HA subunit 1; GST class-alpha member 1; GST-epsilon; GSTA1-1; GTH1; Glutathione S-transferase A1, N-terminally processed; GSTA1

Gene Name

GSTA1

Gene ID

2938&2939&2940&2941&221357

UniProt

P08263

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GSTA1/A2/A3/A4/A5, different from the related mouse and rat sequences by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Liver.

Applications

IF, IHC, WB

Field of Research

Cytokines, Cytoskeleton/ECM, Embryonic Stem Cells, Extracellular Matrix, Signal Transduction, Stem Cells, Structures

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

References & Citations

1. Atkinson, HJ; Babbitt, PC (Nov 24, 2009) . Glutathione transferases are structural and functional outliers in the thioredoxin fold.. Biochemistry 48 (46) : 11108–16. 2. Boyer TD (March 1989) . The glutathione S-transferases: an update. Hepatology 9 (3) : 486–96.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25722 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Gremlin-1

Subcellular Location

Cytoplasm.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Glutathione S-transferase A1/A2/A3/A4/A5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.