Anti-HDAC7 Antibody Picoband®

About this product

Boster Bio Anti-HDAC7 Antibody Picoband® catalog # PB9629. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9629

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone deacetylase 7 is an enzyme that in humans is encoded by the HDAC7 gene. It is mapped to 12q13.1. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation / deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Histone deacetylase 7; HD7;3.5.1.98; Histone deacetylase 7A; HD7a; HDAC7; HDAC7A

Gene Name

HDAC7

Gene ID

51564

UniProt

Q8WUI4

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC7, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Liver.

Applications

WB

Field of Research

Acetylation, Cardiovascular, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Hypertrophy, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity) . May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. Positively regulates the transcriptional repressor activity of FOXP3 (PubMed:17360565) . .

References & Citations

1. Entrez Gene: HDAC7A histone deacetylase 7A. 2. Kao HY, Downes M, Ordentlich P, Evans RM (Jan 2000) . Isolation of a novel histone deacetylase reveals that class I and class II deacetylases promote SMRT-mediated repression. Genes & Development 14 (1) : 55–66. 3. Marks PA, Richon VM, Rifkind RA (Aug 2000) . Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. Journal of the National Cancer Institute 92 (15) : 1210–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

102927 MW

Observed Molecular Weight

103 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Histone deacetylase 7

Subcellular Location

Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and is due to its phosphorylation.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Histone deacetylase 7

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.