Anti-Hex/HHEX Antibody Picoband®

About this product

Boster Bio Anti-Hex/HHEX Antibody Picoband® catalog # PB9631. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9631

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hematopoietically-expressed homeobox protein HHEX is a protein that in humans is encoded by the HHEX gene. Homeobox genes are members of a family of transcription factors that regulate tissue development in many different organisms. Hromas et al. (1993) set out to identify homeobox genes that might play a role in hematopoiesis. And using somatic cell hybrid analysis, they mapped the HHEX gene to chromosome 10, where the HOX11 gene is located. Homeobox genes are involved in neoplastic transformation of both epithelial and hemopoietic tissues. The divergent homeobox gene HEX is expressed in the anterior visceral endoderm during early mouse development and in some adult tissues of endodermal origin, including liver and thyroid. D'Elia et al.’s findings suggested that regulation of HEX entry in the nucleus of thyrocytes may represent a critical step during human thyroid tumorigenesis.

Synonyms

Hematopoietically-expressed homeobox protein HHEX; Homeobox protein HEX; Homeobox protein PRH; HHEX; HEX, PRH, PRHX

Gene Name

HHEX

Gene ID

3087

UniProt

Q03014

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Hex, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Liver and promyelocytic leukemia cell line HL- 60. .

Applications

WB

Field of Research

Developmental Biology, Developmental Families, Domain Families, Embryogenesis, Epigenetics and Nuclear Signaling, Lineage Markers, Lineage Specification, Stem Cells, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by ly targeting NODAL (By similarity) . .

References & Citations

1. D'Elia, A. V., Tell, G., Russo, D., Arturi, F., Puglisi, F., Manfioletti, G., Gattei, V., Mack, D. L., Cataldi, P., Filetti, S., Di Loreto, C., Damante, G. Expression and localization of the homeodomain-containing protein HEX in human thyroid tumors. J. Clin. Endocr. Metab. 87: 1376-1383, 2002. 2. Hromas, R., Radich, J., Collins, S. PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells. Biochem. Biophys. Res. Commun. 195: 976-983, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30022 MW

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Hematopoietically-expressed homeobox protein HHEX

Subcellular Location

Nucleus .

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Hematopoietically-expressed homeobox protein Hhex

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.