Anti-GRP94/HSP90B1 Antibody Picoband®

About this product

Boster Bio Anti-GRP94/HSP90B1 Antibody Picoband® catalog # PB9637. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9637

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP) -metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.

Synonyms

Endoplasmin;94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog; HSP90B1; GRP94, TRA1

Gene Name

HSP90B1

Gene ID

7184

UniProt

P14625

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human GRP94 recombinant protein (Position: R43-H221) . Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, ER Proteins, Metabolism, Metabolism Processes, Organelles, Pathways and Processes, Protein Trafficking, Response To Hypoxia, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity) . Functions in endoplasmic reticulum associated degradation (ERAD) . Has ATPase activity. .

References & Citations

1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (December 2005) . The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86 (6) : 627–37. 2. Maki RG, Old LJ, Srivastava PK (August 1990) . Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins. Proc. Natl. Acad. Sci. U.S.A.87 (15) : 5658–62. 3. Randow F, Seed B (2001) . Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability.. Nat. Cell Biol. 3 (10) : 891–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

92469 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Endoplasmin

Subcellular Location

Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Endoplasmin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.