Anti-IRF5 Antibody Picoband®

About this product

Boster Bio Anti-IRF5 Antibody Picoband® catalog # PB9646. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9646

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interferon regulatory factor 5, also called IRF5 or SLEB10, is a protein that in humans is encoded by the IRF5 gene. IRF5 gene is mapped to 7q32.1. This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. This gene is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection.

Synonyms

Interferon regulatory factor 5; IRF-5; IRF5

Gene Name

IRF5

Gene ID

3663

UniProt

Q13568

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human IRF5, different from the related mouse sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed throughout the epithelium of the colon. Also expressed in lamina propria. .

Applications

IHC, WB

Field of Research

Cytokines, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Immunology, Innate Immunity, Interferons, Polymerase Associated Factors, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. .

References & Citations

1. Couzinet, A., Tamura, K., Chen, H., Nishimura, K., Wang, Z., Morishita, Y., Takeda, K., Yagita, H., Yanai, H., Taniguchi, T., Tamura, T. A cell-type-specific requirement for IFN regulatory factor 5 (IRF5) in Fas-induced apoptosis. Proc. Nat. Acad. Sci. 105: 2556-2561, 2008. 2. Sigurdsson, S., Goring, H. H. H., Kristjansdottir, G., Milani, L., Nordmark, G., Sandling, J. K., Eloranta, M.-L., Feng, D., Sangster-Guity, N., Gunnarsson, I., Svenungsson, E., Sturfelt, G., Jonsen, A., Truedsson, L., Barnes, B. J., Alm, G., Ronnblom, L., Syvanen, A.-C. Comprehensive evaluation of the genetic variants of interferon regulatory growth factor 5 (IRF5) reveals a novel 5 bp length polymorphism as strong risk factor for systemic lupus erythematosus. Hum. Molec. Genet. 17: 872-881, 2008.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

56044 MW

Observed Molecular Weight

56 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Interferon regulatory factor 5

Subcellular Location

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Interferon regulatory factor 5

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.