Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband®

About this product

Boster Bio Anti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband® catalog # PB9647. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9647

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. It is mapped to 17q21.32. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Synonyms

Integrin alpha-IIb; GPalpha IIb; GPIIb; Platelet membrane glycoprotein IIb; CD41; Integrin alpha-IIb heavy chain; Integrin alpha-IIb light chain, form 1; Integrin alpha-IIb light chain, form 2; ITGA2B; GP2B, ITGAB

Gene Name

ITGA2B

Gene ID

3674

UniProt

P08514

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ITGA2B, different from the related mouse sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U-937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.

Applications

IHC, WB

Field of Research

Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Platelets, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

References & Citations

1. Entrez Gene: ITGA2B integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) . 2. Naik UP, Parise LV (1997) . Structure and function of platelet alpha IIb beta 3.. Curr. Opin. Hematol. 4 (5) : 317–22. 3. Quinn MJ, Byzova TV, Qin J et al. (2004) . Integrin alphaIIbbeta3 and its antagonism.. Arterioscler. Thromb. Vasc. Biol. 23 (6) : 945–52.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

113377 MW

Observed Molecular Weight

120-135 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin alpha-IIb

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Integrin alpha-Iib

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.