Anti-KCNA3 Antibody Picoband®

About this product

Boster Bio Anti-KCNA3 Antibody Picoband® catalog # PB9650. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9650

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Potassium voltage-gated channel, shaker-related subfamily, member 3, also known as KCNA3 or Kv1.3, is a protein that in humans is encoded by the KCNA3 gene. This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T-cell proliferation and activation. This gene appears to be intronless and it is clustered together with KCNA2 and KCNA10 genes on chromosome 1. And Kv1.3 has been reported to be expressed in the inner mitochondrial membrane in lymphocytes. The apoptotic protein Bax has been suggested to insert into theouter mitochondrial membrane and occlude the pore of Kv1.3 via a lysine residue. Thus, Kv1.3 modulation may be one of many mechanisms that contribute to apoptosis.

Synonyms

Potassium voltage-gated channel subfamily A member 3; HGK5; HLK3; HPCN3; Voltage-gated K (+) channel HuKIII; Voltage-gated potassium channel subunit Kv1.3; KCNA3; HGK5

Gene Name

KCNA3

Gene ID

3738

UniProt

P22001

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human KCNA3, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Detected in brain cortex (PubMed:16473933) . Detected in peroneal nerve in the juxtaparanodal regions of the node of Ranvier; expression is decreased in patients with diabetes mellitus that suffer from axonal neuropathy (PubMed:22649228) . Detected in paranodal and juxtanodal zones in myelinated spinal cord (at protein level) (PubMed:11086297) . .

Applications

WB

Field of Research

Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

References & Citations

1. Szabó I, Bock J, Grassmé H, Soddemann M, Wilker B, Lang F, Zoratti M, Gulbins E (September 2008) . Mitochondrial potassium channel Kv1.3 mediates Bax-induced apoptosis in lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 105 (39) : 14861–6. 2. Storey NM, Gómez-Angelats M, Bortner CD, Armstrong DL, Cidlowski JA (August 2003) . Stimulation of Kv1.3 potassium channels by death receptors during apoptosis in Jurkat T lymphocytes. J. Biol. Chem. 278 (35) : 33319–26.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

63842 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Potassium voltage-gated channel subfamily A member 3

Subcellular Location

Cell membrane ; Multi-pass membrane protein.

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Potassium voltage-gated channel subfamily A member 3

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.