- Catalog number
- PB9657
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al. (1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed 'reverse cholesterol transport.' The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein (HDL) . Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver (Jonas, 2000) .
- Synonyms
- Phosphatidylcholine-sterol acyltransferase;2.3.1.43 ; Lecithin-cholesterol acyltransferase; Phospholipid-cholesterol acyltransferase; Lcat
- Gene Name
- Lcat
- Gene ID
- 16816
- UniProt
- P16301
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse LCAT, different from the related human sequence by six amino acids, and from the related rat sequence by four amino acids.
- Clonality
- Polyclonal
- Tissue Specificity
- Detected in blood plasma (PubMed:8820107) . Produced and secreted by astrocytes (at protein level) (PubMed:19065001) . Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. .
- Applications
- WB
- Field of Research
- Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:19065001) . The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed:19065001) . Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed:19065001) . Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:19065001) . Has a preference for plasma 16:0-18:2 or 18:O- 18:2 phosphatidylcholines (PubMed:8820107) . .
- References & Citations
- 1. Azoulay, M., Henry, I., Tata, F., Weil, D., Grzeschik, K. H., Chaves, E., McIntyre, N., Williamson, R., Humphries, S. E., Junien, C. The structural gene for lecithin:cholesterol acyl transferase (LCAT) maps to 16q22. Ann. Hum. Genet. 51: 129-136, 1987. 2. Frohlich, J., McLeod, R., Hon, K. Lecithin:cholesterol acyl transferase (LCAT) . Clin. Biochem. 15: 269-278, 1982. 3. Jonas, A. Lecithin cholesterol acyltransferase. Biochim. Biophys. Acta 1529: 245-256, 2000.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 49747 MW
- Observed Molecular Weight
- 50 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Phosphatidylcholine-sterol acyltransferase
- Subcellular Location
- Secreted . Secreted into blood plasma (PubMed:8820107) . Produced in astrocytes and secreted into cerebral spinal fluid (CSF) (By similarity) . .
- Sequence Similarities
- Contains 1 HMG box DNA-binding domain.
- Protein Name
- Phosphatidylcholine-sterol acyltransferase
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
