- Catalog number
- PB9658
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.
- Synonyms
- Leptin; Obesity factor; Lep; Ob
- Gene Name
- Lep
- Gene ID
- 16846
- UniProt
- P41160
- Host
- Rabbit
- Reactivity
- Mouse
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin, different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.
- Clonality
- Polyclonal
- Tissue Specificity
- Detected in blood plasma (PubMed:8820107) . Produced and secreted by astrocytes (at protein level) (PubMed:19065001) . Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. .
- Applications
- ELISA, WB
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Diabetes-associated, Growth Factors/Hormones, Heart Disease, Mesenchymal Stem Cells, Metabolism, Neurology Process, Neuroscience, Obesity, Signal Transduction, Stem Cells
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act ly or inly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
- References & Citations
- 1. Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallone RL, Burley SK, Friedman JM (July 1995) . Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269 (5223) : 543–6. 2. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S (November 1995) . Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat. Med. 1 (11) : 1155–61. 3. Pan H, Guo J, Su Z (May 2014) . Advances in understanding the interrelations between leptin resistance and obesity.Physiology & Behavior 130: 157–169.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 18709 MW
- Observed Molecular Weight
- 16 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Leptin
- Subcellular Location
- Secreted .
- Sequence Similarities
- Contains 1 HMG box DNA-binding domain.
- Protein Name
- Leptin
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
