Anti-Mesothelin/MSLN Antibody

About this product

Boster Bio Anti-Mesothelin/MSLN Antibody Picoband® catalog # PB9671. Tested in IHC applications. This antibody reacts with Mouse.

Specifications

Catalog number

PB9671

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Synonyms

Mesothelin; Pre-pro-megakaryocyte-potentiating factor; Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form; Msln; Mes, Mpf

Gene Name

Msln

Gene ID

56047

UniProt

Q61468

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576) . Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in lung and heart. Expressed at low levels in spleen, liver, kidney and testis. Present in lung (at protein level) . .

Applications

IHC

Field of Research

Cancer, Cell Type Markers, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Membrane-anchored forms may play a role in cellular adhesion. .

References & Citations

1. Bera, T. K., Pastan, I. Mesothelin is not required for normal mouse development or reproduction. Molec. Cell. Biol. 20: 2902-2906, 2000. 2. Scholler, N., Fu, N., Yang, Y., Ye, Z., Goodman, G. E., Hellstrom, K. E., Hellstrom, I. Soluble member (s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proc. Nat. Acad. Sci. 96: 11531-11536, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

69423 MW

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mesothelin

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor . Golgi apparatus .

Protein Name

Mesothelin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.