Anti-CARD12/NLRC4 Antibody Picoband®

About this product

Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9673

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.

Synonyms

NLR family CARD domain-containing protein 4; CARD, LRR, and NACHT-containing protein; Clan protein; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf; NLRC4; CARD12, CLAN, CLAN1, IPAF; UNQ6189/PRO20215

Gene Name

NLRC4

Gene ID

58484

UniProt

Q9NPP4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Applications

IF, ICC, WB

Field of Research

Apoptosis, Cancer, Caspases, Cell Biology, Cell Cycle, Cell Death, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Intracellular, Macrophage/Inflammation, Metabolism, Metabolism Processes, Nuclear, Pathways and Processes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Key component of inflammasomes that inly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. .

References & Citations

1. Entrez Gene: NLRC4 NLR family, CARD domain containing 4. 2. Geddes BJ, Wang L, Huang WJ, Lavellee M, Manji GA, Brown M, Jurman M, Cao J, Morgenstern J, Merriam S, Glucksmann MA, DiStefano PS, Bertin J (Jun 2001) . Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochemical and Biophysical Research Communications284 (1) : 77–82. 3. Hu Z, Yan C, Liu P, Huang Z, Ma R, Zhang C, Wang R, Zhang Y, Martinon F, Miao D, Deng H, Wang J, Chang J, Chai J (Jul 2013) . Crystal structure of NLRC4 reveals its autoinhibition mechanism. Science 341 (6142) : 172–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

116159 MW

Observed Molecular Weight

116 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

NLR family CARD domain-containing protein 4

Subcellular Location

Cytoplasm . Cytoplasm, cytosol . Cytoplasmic filaments.

Protein Name

NLR family CARD domain-containing protein 4

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.