Anti-GRK3/ADRBK2 Antibody Picoband®

About this product

Boster Bio Anti-GRK3/ADRBK2 Antibody Picoband® catalog # PB9682. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9682

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Beta-adrenergic receptor kinase 2  (beta-ARK-2), also known as  G-protein-coupled receptor kinase 3  (GRK3), is an  enzyme  that in humans is encoded by the  ADRBK2  gene. The human  ADRBK2  gene is located on 22q11. The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.

Synonyms

Beta-adrenergic receptor kinase 2; Beta-ARK-2;2.7.11.15; G-protein-coupled receptor kinase 3; ADRBK2; BARK2, GRK3

Gene Name

ADRBK2

Gene ID

157

UniProt

P35626

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GRK3, different from the related mouse and rat sequences by five amino acids.

Clonality

Polyclonal

Tissue Specificity

Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Ser/Thr Kinases, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

References & Citations

1. Benovic, J. L., Onorato, J. J., Arriza, J. L., Stone, W. C., Lohse, M., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Caron, M. G., Lefkowitz, R. J. Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2: a new member of the receptor kinase family. J. Biol. Chem. 266: 14939-14946, 1991. 2. Calabrese G, Sallese M, Stornaiuolo A, Stuppia L, Palka G, De Blasi A (Feb 1995) . Chromosome mapping of the human arrestin (SAG), beta-arrestin 2 (ARRB2), and beta-adrenergic receptor kinase 2 (ADRBK2) genes. Genomics 23 (1) : 286–8. 3. Parruti, G., Ambrosini, G., Sallese, M., De Blasi, A. Molecular cloning, functional expression and mRNA analysis of human beta-adrenergic receptor kinase 2. Biochem. Biophys. Res. Commun. 190: 475-481, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

79710 MW

Observed Molecular Weight

80 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Beta-adrenergic receptor kinase 2

Subcellular Location

Secreted.

Protein Name

Beta-adrenergic receptor kinase 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.