Anti-Tissue Factor/F3 Antibody Picoband®

About this product

Boster Bio Anti-Tissue Factor/F3 Antibody Picoband® catalog # PB9702. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9702

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tissue factor also called platelet tissue factor, ?factor III, or?CD142. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

Synonyms

Tissue factor; TF; Coagulation factor III; CD142; F3; Cf-3, Cf3

Gene Name

F3

Gene ID

14066

UniProt

P20352

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Tissue Factor recombinant protein (Position: A29-A294) . Mouse Tissue Factor shares 62.2% and 82.1% amino acid (aa) sequence identity with human and rat Tissue Factor, respectively.

Clonality

Polyclonal

Tissue Specificity

Lung, placenta and pancreas. .

Applications

WB

Field of Research

Cardiovascular

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

References & Citations

1. Entrez Gene: F3 coagulation factor III (thromboplastin, tissue factor) . 2. Bogdanov VY, Balasubramanian V, Hathcock J, Vele O, Lieb M, Nemerson Y (April 2003) . Alternatively spliced human tissue factor: a circulating, soluble, thrombogenic protein. Nat. Med. 9 (4) : 458–62. 3. Mackman N (1995) . Regulation of the tissue factor gene. FASEB J. 9 (10) : 883–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32935 MW

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tissue factor

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Tissue factor

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.