Anti-FMRP/FMR1 Antibody Picoband®

About this product

Boster Bio Anti-FMRP/FMR1 Antibody Picoband® catalog # PB9703. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9703

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

FMR1  (fragile X mental retardation 1) is a  human  gene  that codes for a  protein  called  fragile X  mentalretardation  protein, or FMRP. This protein, most commonly found in the brain, is essential for normal  cognitive development  and female reproductive function. Mutations of this gene can lead to  fragile X syndrome, mental retardation,   premature ovarian failure,   autism,   Parkinson's disease, developmental delays and other cognitive deficits. The protein encoded by this gene binds RNA and is associated with polysomes. Additionally, the encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) . Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

Synonyms

Fragile X mental retardation protein 1; FMRP; Protein FMR-1; FMR1

Gene Name

FMR1

Gene ID

2332

UniProt

Q06787

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FMRP, different from the related mouse and rat sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. .

Applications

IF, IHC, ICC, WB

Field of Research

Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Neural Signal Transduction, Neurology Process, Neuroscience

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity) . RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly (G), binds moderately to poly (U) but shows very little binding to poly (A) or poly (C) . .

References & Citations

1. Verkerk AJ, Pieretti M, Sutcliffe JS, Fu YH, Kuhl DP, Pizzuti A, Reiner O, Richards S, Victoria MF, Zhang FP (May 1991) . Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome. Cell 65 (5) : 905–14. 2. Verheij C, Bakker CE, de Graaff E, Keulemans J, Willemsen R, Verkerk AJ, Galjaard H, Reuser AJ, Hoogeveen AT, Oostra BA (June 1993) . Characterization and localization of the FMR-1 gene product associated with fragile X syndrome. Nature 363 (6431) : 722–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71174 MW

Observed Molecular Weight

71-73 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Fragile X mental retardation protein 1

Subcellular Location

Cytoplasm . Nucleus . Nucleus, nucleolus .

Protein Name

Synaptic functional regulator FMR1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.