Anti-FXYD1 Antibody Picoband®

About this product

Boster Bio Anti-FXYD1 Antibody Picoband® catalog # PB9704. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9704

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Phospholemman?is a?protein?that in humans is encoded by the?FXYD1?gene. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. Transcript variants with different 5' UTR sequences have been described in the literature.

Synonyms

Phospholemman; FXYD domain-containing ion transport regulator 1; FXYD1; PLM

Gene Name

FXYD1

Gene ID

5348

UniProt

O00168

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human FXYD1 recombinant protein (Position: E21-R92) . Human FXYD1 shares 86.1% and 87.5% amino acid (aa) sequence identity with mouse and rat FXYD1, respectively.

Clonality

Polyclonal

Tissue Specificity

Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.

Applications

IHC, WB

Field of Research

ATPases, Cancer, Cardiovascular, Channels, Contractility, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.

References & Citations

1. Entrez Gene: FXYD1 FXYD domain containing ion transport regulator 1 (phospholemman) . 2. Chen LS, Lo CF, Numann R, Cuddy M (Jul 1997) . Characterization of the human and rat phospholemman (PLM) cDNAs and localization of the human PLM gene to chromosome 19q13.1. Genomics 41 (3) : 435–43.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10441 MW

Observed Molecular Weight

10 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Phospholemman

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Phospholemman

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.