Anti-GLP1/GCG Antibody

About this product

Boster Bio Anti-GLP1/GCG Antibody Picoband® catalog # PB9705. Tested in IF, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

PB9705

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.?It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.?It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

Synonyms

Glucagon; Glicentin; Glicentin-related polypeptide; GRPP; Oxyntomodulin; OXM; OXY; Glucagon; Glucagon-like peptide 1; GLP-1; Incretin hormone; Glucagon-like peptide 1 (7-37) ; GLP-1 (7-37) ; Glucagon-like peptide 1 (7-36) ; GLP-1 (7-36) ; Glucagon-like peptide 2; GLP-2; GCG

Gene Name

GCG

Gene ID

2641

UniProt

P01275

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GLP1, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

Applications

IF, IHC

Field of Research

Cancer, Cancer Metabolism, Developmental Biology, Energy Transfer Pathways, Growth Factors/Hormones, Integration Of Energy, Integration Of Energy Metabolism, Lineage Markers, Lineage Specification, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Stem Cells, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.

References & Citations

1. Arora S, Galich P (Mar 2009) . Myth: glucagon is an effective first-line therapy for esophageal foreign body impaction. Cjem 11 (2) : 169–71. 2. Holz, G. G., IV, Kuhtreiber, W. M., Habener, J. F. Pancreatic beta-cells are rendered glucose-competent by the insulinotropic hormone glucagon-like peptide-1 (7-37) . Nature 361: 362-365, 1993. 3. Liljenquist JE, Bomboy JD, Lewis SB, Sinclair-Smith BC, Felts PW, Lacy WW, Crofford OB, Liddle GW (Jan 1974) . Effects of glucagon on lipolysis and ketogenesis in normal and diabetic men (PDF) . The Journal of Clinical Investigation 53 (1) : 190–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20909 MW

Observed Molecular Weight

10 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glucagon

Subcellular Location

Secreted.

Protein Name

Glucagon

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.