Anti-GRK6 Antibody Picoband®

About this product

Boster Bio Anti-GRK6 Antibody Picoband® catalog # PB9709. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9709

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

G protein-coupled receptor kinase 6?is an?enzyme?that in humans is encoded by the?GRK6?gene. It is mapped to 5q35. This gene encodes a member of the guanine nucleotide-binding protein (G protein) -coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. Also, GRK6 appears to be involved in responses to?morphine.

Synonyms

G protein-coupled receptor kinase 6;2.7.11.16; G protein-coupled receptor kinase GRK6; GRK6; GPRK6

Gene Name

GRK6

Gene ID

2870

UniProt

P43250

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GRK6, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

WB

Field of Research

Cardiovascular, G Protein Signaling, Hypertrophy, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity) . Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro) . .

References & Citations

1. Entrez Gene: GRK6 G protein-coupled receptor kinase 6. 2. Haribabu B, Snyderman R (Nov 1993) . Identification of additional members of human G-protein-coupled receptor kinase multigene family. Proc Natl Acad Sci U S A 90 (20) : 9398–402. 3. Raehal KM, Schmid CL, Medvedev IO, Gainetdinov RR, Premont RT, Bohn LM (June 2009) . Morphine-Induced Physiological and Behavioral Responses in Mice Lacking G Protein-Coupled Receptor Kinase 6. Drug and Alcohol Dependence 104 (3) : 187–96.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

65991 MW

Observed Molecular Weight

66 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

G protein-coupled receptor kinase 6

Subcellular Location

Membrane; Lipid-anchor.

Protein Name

G protein-coupled receptor kinase 6

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.