Anti-LIM kinase 2/LIMK2 Antibody Picoband®

About this product

Boster Bio Anti-LIM kinase 2/LIMK2 Antibody Picoband® catalog # PB9717. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9717

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LIM domain kinase 2?is an?enzyme?that in humans is encoded by the?LIMK2?gene. There are approximately 40 known eukaryotic LIM proteins, so named for the?LIM domains?they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Synonyms

LIM domain kinase 2; LIMK-2;2.7.11.1; LIMK2

Gene Name

LIMK2

Gene ID

3985

UniProt

P53671

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LIM kinase 2, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. .

Applications

WB

Field of Research

Actin, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. .

References & Citations

1. Entrez Gene: LIMK2 LIM domain kinase 2. 2. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (Dec 1999) . The DNA sequence of human chromosome 22.Nature 402 (6761) : 489–95. 3. Okano I, Hiraoka J, Otera H, Nunoue K, Ohashi K, Iwashita S, Hirai M, Mizuno K (Dec 1995) . Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs. The Journal of Biological Chemistry 270 (52) : 31321–30.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72232 MW

Observed Molecular Weight

72 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

LIM domain kinase 2

Subcellular Location

Isoform LIMK2a: Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus.

Protein Name

LIM domain kinase 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.