Anti-HSD11B2 Antibody Picoband®

About this product

Boster Bio Anti-HSD11B2 Antibody catalog # RP1094. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1094

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Corticosteroid 11-β-dehydrogenase isozyme 2, also known as?11-β-hydroxysteroid dehydrogenase 2, ?is an enzyme?that in humans is encoded by the?HSD11B2?gene. There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

Synonyms

Corticosteroid 11-beta-dehydrogenase isozyme 2;1.1.1.-;11-beta-hydroxysteroid dehydrogenase type 2;11-DH2;11-beta-HSD2;11-beta-hydroxysteroid dehydrogenase type II;11-HSD type II;11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase;11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3; HSD11B2; HSD11K , SDR9C3

Gene Name

HSD11B2

Gene ID

3291

UniProt

P80365

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSD11B2, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in kidney, pancreas, prostate, ovary, small intestine and colon. At midgestation, expressed at high levels in placenta and in fetal kidney and, at much lower levels, in fetal lung and testis (PubMed:8530071) . .

Applications

IF, IHC, IHC-F, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Growth Factors/Hormones, Heart Disease, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

References & Citations

1. Albiston AL, Obeyesekere VR, Smith RE, Krozowski ZS (November 1994) . Cloning and tissue distribution of the human 11 beta-hydroxysteroid dehydrogenase type 2 enzyme. Mol. Cell. Endocrinol. 105 (2) : R11–7. 2. Brown RW, Chapman KE, Kotelevtsev Y, Yau JL, Lindsay RS, Brett L, Leckie C, Murad P, Lyons V, Mullins JJ, Edwards CR, Seckl JR (February 1996) . Cloning and production of antisera to human placental 11 beta-hydroxysteroid dehydrogenase type 2. Biochem. J. 313 (Pt 3) (Pt 3) : 1007–17.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44127 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Corticosteroid 11-beta-dehydrogenase isozyme 2

Subcellular Location

Microsome . Endoplasmic reticulum .

Protein Name

Corticosteroid 11-beta-dehydrogenase isozyme 2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.