Anti-PU.1/Spi1 Antibody

About this product

Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

RP1097

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transcription factor PU.1;31 kDa-transforming protein; SPI1

Gene Name

SPI1

Gene ID

6688

UniProt

P17947

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196) . Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.

Clonality

Polyclonal

Applications

IHC

Field of Research

Adaptive Immunity, B Cells, DNA/RNA, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Non-CD, RNA Processing, Splicing, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) . .

References & Citations

1. Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1. 2. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998) . The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS. J. Biol. Chem. 273 (9) : 4838–42. 3. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990) . The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors. Oncogene 5 (5) : 663–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31083 MW

Observed Molecular Weight

150 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Transcription factor PU.1

Subcellular Location

Nucleus .

Protein Name

Transcription factor PU.1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.