- Catalog number
- PB9754
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- BAG family molecular chaperone regulator 3 (BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men.
- Synonyms
- BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1; BAG3; BIS
- Gene Name
- BAG3
- Gene ID
- 9531
- UniProt
- O95817
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human Bag3 recombinant protein (Position: H100-N561) . Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. .
- Applications
- IHC, WB
- Field of Research
- Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
- References & Citations
- 1. Arndt V, Dick N, Tawo R, Dreiseidler M, Wenzel D, Hesse M, Fürst DO, Saftig P, Saint R, Fleischmann BK, Hoch M, Höhfeld J (January 2010) . Chaperone-assisted selective autophagy is essential for muscle maintenance. Curr Biol 20 (2) : 143–8. 2. Homma, S., Iwasaki, M., Shelton, G. D., Engvall, E., Reed, J. C., Takayama, S. BAG3 deficiency results in fulminant myopathy and early lethality. Am. J. Path. 169: 761-773, 2006. 3. Villard, E; Perret, C, Gary, F, Proust, C, Dilanian, G, Hengstenberg, C, Ruppert, V, Arbustini, E, Wichter, T, Germain, M, Dubourg, O, Tavazzi, L, Aumont, MC, DeGroote, P, Fauchier, L, Trochu, JN, Gibelin, P, Aupetit, JF, Stark, K, Erdmann, J, Hetzer, R, Roberts, AM, Barton, PJ, Regitz-Zagrosek, V; Cardiogenics Consortium, Aslam, U, Duboscq-Bidot, L, Meyborg, M, Maisch, B, Madeira, H, Waldenström, A, Galve, E, Cleland, JG, Dorent, R, Roizes, G, Zeller, T, Blankenberg, S, Goodall, AH, Cook, S, Tregouet, DA, Tiret, L, Isnard, R, Komajda, M, Charron, P, Cambien, F (April 1, 2011) . A genome-wide association study identifies two loci associated with heart failure due to dilated cardiomyopathy 32 (9) . pp. 1065–76.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 61595 MW
- Observed Molecular Weight
- 80 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- BAG family molecular chaperone regulator 3
- Subcellular Location
- Cytoplasm, cytoskeleton . Endosome . Cytoplasmic vesicle, secretory vesicle, acrosome . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendrite . Cell projection, dendritic spine . Cell junction, synapse . Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity) . Enriched on the plasma membrane. .
- Protein Name
- BAG family molecular chaperone regulator 3
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
