Anti-eIF4A2 Antibody Picoband®

About this product

Boster Bio Anti-eIF4A2 Antibody Picoband® catalog # PB9758. Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9758

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic initiation factor 4A-II is a protein that in humans is encoded by the EIF4A2 gene. It is mapped to 18p11.2. Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation.

Synonyms

Eukaryotic initiation factor 4A-II; eIF-4A-II; eIF4A-II;3.6.4.13; ATP-dependent RNA helicase eIF4A-2; Eukaryotic initiation factor 4A-II, N-terminally processed; EIF4A2; DDX2B, EIF4F

Gene Name

EIF4A2

Gene ID

1974

UniProt

Q14240

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed. .

Applications

Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Translation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

References & Citations

1. Entrez Gene: EIF4A2 eukaryotic translation initiation factor 4A, isoform 2. 2. Meijer, H. A., Kong, Y. W., Lu, W. T., Wilczynska, A., Spriggs, R. V., Robinson, S. W., Godfrey, J. D., Willis, A. E., Bushell, M. Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation. Science 340: 82-85, 2013. 3. Sudo K, Takahashi E, Nakamura Y (Jan 1996) . Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2. Cytogenet Cell Genet 71 (4) : 385–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46402 MW

Observed Molecular Weight

49 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Eukaryotic initiation factor 4A-II

Subcellular Location

Nucleus . Accumulates at sites of DNA damage following UV irradiation.

Protein Name

Eukaryotic initiation factor 4A-II

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.