Anti-PGK1 Antibody Picoband®

About this product

Boster Bio Anti-PGK1 Antibody Picoband® catalog # PB9774. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9774

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Synonyms

Phosphoglycerate kinase 1;2.7.2.3; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; PGK1; PGKA; MIG10, OK/SW-cl.110

Gene Name

PGK1

Gene ID

5230

UniProt

P00558

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PGK1, different from the related mouse and rat sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous; highest levels of expression in heart and skeletal muscle. .

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) .

References & Citations

1. Entrez Gene: PGK1 phosphoglycerate kinase 1. 2. Michelson AM, Markham AF, Orkin SH (May 1983) . Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase. Proc Natl Acad Sci U S A 80 (2) : 472–6. 3. Singer-Sam J, Keith DH, Tani K, Simmer RL, Shively L, Lindsay S, Yoshida A, Riggs AD (May 1985) . Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase]. Gene 32 (3) : 409–17.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44615 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Phosphoglycerate kinase 1

Subcellular Location

Cytoplasm.

Protein Name

Phosphoglycerate kinase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.