Anti-Phospholipase A2/PLA2G4A Antibody Picoband®

About this product

Boster Bio Anti-Phospholipase A2/PLA2G4A Antibody Picoband® catalog # PB9778. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9778

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PLA2G4A (Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al. (1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-ed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al. (1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue (s) . PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands (Skorecki, 1995) .

Synonyms

Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2;3.1.1.4; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase;3.1.1.5; PLA2G4A; CPLA2, PLA2G4

Gene Name

PLA2G4A

Gene ID

5321

UniProt

P47712

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PLA2G4A, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.

Applications

WB

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Heart Disease, Immunology, Innate Immunity, Lipid and Lipoprotein Metabolism, Lipid Signaling, Macrophage/Inflammation, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.

References & Citations

1. Sharp, J. D., Pickard, R. T., Chiou, X. G., Manetta, J. V., Kovacevic, S., Miller, J. R., Varshavsky, A. D., Roberts, E. F., Strifler, B. A., Brems, D. N., Kramer, R. M. Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2. J. Biol. Chem. 269: 23250-23254, 1994. 2. Skorecki, K. L. Personal Communication. Toronto, Canada 4/19/1995. 3. Tay, A., Simon, J. S., Squire, J., Hamel, K., Jacob, H. J., Skorecki, K. Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers. Genomics 26: 138-141, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85239 MW

Observed Molecular Weight

85 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cytosolic phospholipase A2

Subcellular Location

Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.

Protein Name

Cytosolic phospholipase A2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.