Anti-POT1 Antibody Picoband®

About this product

Boster Bio Anti-POT1 Antibody Picoband® catalog # PB9780. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9780

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protection of telomeres protein 1 is a protein that in humans is encoded by the POT1 gene. This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.

Synonyms

Protection of telomeres protein 1; hPot1; POT1-like telomere end-binding protein; POT1

Gene Name

POT1

Gene ID

25913

UniProt

Q9NUX5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human POT1, different from the related mouse sequence by eleven amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. .

Applications

Flow Cytometry, WB

Field of Research

Cardiovascular, Drug Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. .

References & Citations

1. Entrez Gene: POT1 POT1 protection of telomeres 1 homolog (S. pombe) . 2. Baumann P, Cech TR (May 2001) . Pot1, the putative telomere end-binding protein in fission yeast and humans. Science 292 (5519) : 1171–5. 3. Baumann P, Podell E, Cech TR (Oct 2002) . Human Pot1 (Protection of Telomeres) Protein: Cytolocalization, Gene Structure, and Alternative Splicing. Mol Cell Biol 22 (22) : 8079–87.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71442 MW

Observed Molecular Weight

71 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Protection of telomeres protein 1

Subcellular Location

Nucleus . Chromosome, telomere . Colocalizes with telomeric DNA.

Protein Name

Protection of telomeres protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.