Anti-PPT1 Antibody Picoband®

About this product

Boster Bio Anti-PPT1 Antibody Picoband® catalog # PB9781. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9781

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Palmitoyl-protein thioesterase 1 (PPT-1), also known as palmitoyl-protein hydrolase 1, is an enzyme that in humans is encoded by the PPT1 gene. PPT-1 is a member of the palmitoyl protein thioesterase family. The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4) . Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Palmitoyl-protein thioesterase 1; PPT-1;3.1.2.22; Palmitoyl-protein hydrolase 1; PPT1; PPT

Gene Name

PPT1

Gene ID

5538

UniProt

P50897

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PPT1, different from the related mouse and rat sequences by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. .

Applications

Flow Cytometry, IHC, IHC-F, ICC, WB

Field of Research

Cancer, Lipid Metabolism, Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

References & Citations

1. Entrez Gene: palmitoyl-protein thioesterase 1. 2. Hellsten E, Vesa J, Speer MC, Mäkelä TP, Järvelä I, Alitalo K, Ott J, Peltonen L (June 1993) . Refined assignment of the infantile neuronal ceroid lipofuscinosis (INCL, CLN1) locus at 1p32: incorporation of linkage disequilibrium in multipoint analysis. Genomics 16 (3) : 720–5. 3. Vesa J, Hellsten E, Verkruyse LA, Camp LA, Rapola J, Santavuori P, Hofmann SL, Peltonen L (August 1995) . Mutations in the palmitoyl protein thioesterase gene causing infantile neuronal ceroid lipofuscinosis.Nature 376 (6541) : 584–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34193 MW

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Palmitoyl-protein thioesterase 1

Subcellular Location

Lysosome.

Protein Name

Palmitoyl-protein thioesterase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.